Abstract
Cytotoxic Long-chain Alkene and Terpene Isolated from the Methanol Extract of the Air-dried Leaves of Pipturus arborescens C.B. Rob
Charlie A. Lavilla, Jr., Mylene M. Uy, Shinji Ohta
A long-chain alkene (1-hexacosene) and a terpene were obtained from the
bioactive methanol extract of the air-dried leaves of Pipturus arborescens (Link)
C.B. Rob (“Handalamay”) collected from Poblacion, Kapatagan, Lanao del
Norte. The compounds were isolated through extraction with ethanol, sequential
partitioning with water-chloroform and hexane-90% methanol followed by
fractionation and purification with repeated gravity column chromatography
using gradient mixtures of hexane: ethyl acetate (1:1 ratio). The structures of the
isolates were elucidated on the basis of spectroscopic data: 1H-and 13C-NMR,
1H-13C Heteronuclear Multiquantum Correlation (HMQC), 1H-13C Heteronuclear
Multibond Correlation (HMBC), 1H-1H Homonuclear Correlation Spectroscopy
(COSY), Infra-Red Spectroscopy (IR), Ultraviolet-Visible Spectroscopy
(UV-VIS) and Mass Spectrometry (MS). The isolated compounds were shown to
possess moderate cytotoxic activity against the brine shrimp Artemia salina
(Leach).
KEYWORDS: bioactive, chromatography, fractionation, spectroscopy,1-hexacosene
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