Home › Vol 3, No 1 (2014): August  ›  Abstract ›  View Pdf

Cytotoxic Long-chain Alkene and Terpene Isolated from the Methanol Extract of the Air-dried Leaves of Pipturus arborescens C.B. Rob

Charlie A. Lavilla, Jr., Mylene M. Uy, Shinji Ohta

A long-chain alkene (1-hexacosene) and a terpene were obtained from the bioactive methanol extract of the air-dried leaves of Pipturus arborescens (Link) C.B. Rob (“Handalamay”) collected from Poblacion, Kapatagan, Lanao del Norte. The compounds were isolated through extraction with ethanol, sequential partitioning with water-chloroform and hexane-90% methanol followed by fractionation and purification with repeated gravity column chromatography using gradient mixtures of hexane: ethyl acetate (1:1 ratio). The structures of the isolates were elucidated on the basis of spectroscopic data: 1H-and 13C-NMR, 1H-13C Heteronuclear Multiquantum Correlation (HMQC), 1H-13C Heteronuclear Multibond Correlation (HMBC), 1H-1H Homonuclear Correlation Spectroscopy (COSY), Infra-Red Spectroscopy (IR), Ultraviolet-Visible Spectroscopy (UV-VIS) and Mass Spectrometry (MS). The isolated compounds were shown to possess moderate cytotoxic activity against the brine shrimp Artemia salina (Leach).

The PDF file you selected should load here if your Web browser has a PDF reader plug-in installed (for example, a recent version of Adobe Acrobat Reader).

Alternatively, you can also download the PDF file directly to your computer, from where it can be opened using a PDF reader. To download the PDF, click the Download link below.

If you would like more information about how to print, save, and work with PDFs, Highwire Press provides a helpful Frequently Asked Questions about PDFs.

© 2014 MU Research & Publication Office, Phone: +6388 521 0367 loc 106 | research@mu.edu.ph
85924 Total Visitors