Home › Vol 7, No 2 (2018): December  ›  Abstract ›  View Pdf

Effects of DNA Inserts on the Viscoelasticity of DNA Gel Composites

May Noren D. Nanud1 and Rommel G. Bacabac2

Understanding the viscoelastic phase separation of a DNA mixture will help model soft gels composed of discrete length distribution. The purpose of this study was to investigate the effects of DNA inserts on the viscoelasticity of DNA gel composites. The study focused on determining the viscoelasticity of mixed systems composed of longer double-stranded deoxyribonucleic acid (ds-DNA) solution as a background matrix re-constituted with shorter ds-DNA inserts of controlled lengths. Passive microrheology by video microscopy was employed to characterize shear moduli variations. The total concentration of each mixed system was 0.201 g/L. An increase in the viscous and elastic moduli of the three mixed systems was observed suggesting an enhancement in the viscoelastic properties of the mixed system upon the addition of the inserts. There was a greater enhancement on the viscous and elastic moduli of the system with longer inclusions. The findings of this study may shed light on biopolymer physics that can aid in examining diseases with genetic materials of resolvable length distributions.

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